面筋蛋白外啡肽B5对传染性支气管炎灭活疫苗免疫效果的影响

doi:10.11843/j.issn.0366-6964.2016.03.020

Abstract

Abstract:

Gluten exorphin B5 (GE-B5) was a food-derived opioid peptide identified in vitro in enzymatic digests of wheat gluten，and it was a good candidate of functional feed additives and adjuvant for vaccine development because of its immune function.In this study，we chemically synthesized GE-B5，and then added it to vaccine against infectious bronchitis virus (IBV) H120 strain to immunize chickens for evaluating their growth performance，immunogenicity and protective efficacy.On 0，14，and 21 dpi，the changes in proliferation of peripheral blood mononuclear cells (PBMC) and serum antibody titer were evaluated respectively by MTT method and hemagglutination inhibition assay.The results showed that administration of GE-B5 significantly raised anti-IB antibody titer in the chicken serum and also markedly promoted PBMCs proliferation.Following challenge with IBV Mass 41 strain，chickens inoculated with GE-B5 showed fewer and less severe clinical signs，lower death rate，lower mortality and showed higher protection，as compared to those of the control groups.The result of pathological tissue section showed that the lung and kidney became shorter pathological phenomenon than the control group.This study confirmed that in addition to its immunoregulatory activity and immunopromoting effects，GE-B5 can also promote weight gain.These data have important implications for functional feed additives and novel adjuvants designed for future vaccines.

CLC Number:

S858.315.3

ZHOU Ye-fei，ZHOU Mei-xian. Gluten Exorphin B5 Enhances Immunogenicity and Protective Efficacy of Infectious Bronchitis Virus Vaccine in Broilers[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 566-583.

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Gluten Exorphin B5 Enhances Immunogenicity and Protective Efficacy of Infectious Bronchitis Virus Vaccine in Broilers

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